摘要
目的:星点设计-效应面法优选北五味子果实的提取工艺。方法:以乙醇体积分数、提取时间、溶媒比为自变量,以北五味子总木脂素含量得率为因变量,通过对自变量各水平的多元线性回归及二项式拟合,用效应面法选取最佳工艺,并进行预测分析。结果:确定最优提取工艺为20倍量85%乙醇,提取3次,每次170 min,提取预测值与理论值偏差为0.19%,二项式拟合复相关系数r=0.994 2。结论:星点设计-效应面法优化的北五味子果实提取工艺,方法简单,精密度高,可预测性较优。
Objective:To optimize extraction process of Schisandra chinensis fruit by central composite design/response surface method.Method: Independent variables were ethanol concentration,extraction time and solvent ratio,dependent variable was extraction rate of total saponins in S.chinensis.Linear or no-linear mathematic models were used to estimate relationship between independent and dependent variables.Response surface methodology was used to optimize extraction process,prediction was carried out through comparing observed and predicted values.Result: Optimal conditions of extraction process were as following:extracted 3 times with 85% ethanol for 170 min each time.The bias between observed and predicted values was 0.19%.Regression coefficients of binomial fitting complex model was as high as 0.994 2.Conclusion: This extraction process of S.chinensis fruit by central composite design/response surface methodology was simple,high precision,good predictability.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第23期28-31,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
北五味子
星点设计
效应面优化法
总木脂素
提取工艺
Schisandra chinensis
central composite design
response surface method
total lignans
extraction process