摘要
目的:建立反相高效液相色谱法同时测定不同根龄虎杖根中虎杖苷和白藜芦醇含量。方法:采用Kromasil C18(4.6 mm×250 mm,5μm)色谱柱,流动相甲醇-1.0%磷酸水溶液(50∶50),流速0.8 mL.min-1,检测波长319 nm,柱温为30℃。结果:虎杖苷和白藜芦醇的进样量分别在0.716～7.160μg(r=0.999 6)和0.740～7.400μg(r=0.999 8)与峰面积呈良好的线性关系;平均回收率(n=6)分别为99.7%,98.5%。结论:本方法快速简便,重复性好,专属性强,可作为虎杖根的质量控制方法。
Objective：To develop a reserved-phase HPLC method for the content determination of polygonin and resveratrol in different age root of Polygonum cuspidatum.Method： The analysis was carried out with Kromasil C18（4.6 mm×250 mm,5 μm） column and the mobile phase consisted of acetonitrile-1.0% phosphoric acid aqueous solution（50∶50） at a flow rate of 0.8 mL·min-1.The detection wavelength was 319 nm and the column temperature was set at 30 ℃.Result： The calibration curves were linear within the range of 0.716-7.160 μg（r=0.999 6） for polygonin and 0.740-7.400 μg（r=0.999 8） for resveratrol.The average recoveries were 99.7% for erbascoside and 98.5% for resveratrol.Conclusion： The method is simple,accurate,reproducible and precise,and applicable for the determination of polygonin and resveratrol in different age root of Polygonum cuspidatum.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第23期62-64,共3页
Chinese Journal of Experimental Traditional Medical Formulae
