HPLC测定咽扁宁冲剂中梓醇和肉桂酸含量

Determination of Catalpol and Cinnamicacid in Yanbianning Granule by HPLC

目的:建立咽扁宁冲剂中梓醇和肉桂酸的含量测定方法。方法:采用Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm),梓醇:流动相为水-甲醇-乙腈(95∶4∶1),波长212 nm,流速1.0 mL.min-1;肉桂酸:流动相为甲醇-1%冰乙酸溶液(50∶50),波长278 nm,流速1.2 mL.min-1。结果:梓醇和肉桂酸分别在3.36～168,0.156～104 mg.L-1呈良好的线性关系。平均回收率分别为梓醇101.59%,RSD 1.36%(n=5);肉桂酸101.28%,RSD 1.28%(n=5)。结论:此方法简便,准确,重复性好,可用于咽扁宁冲剂的质量控制和评价。

Objective：To establish the method for determination of catalpol and cinnamicacid in Yanbianning granule.Method： Catalpol and cinnamicacid were determined by HPLC on C18-column at 212 nm and 278 nm,with water-methanol-acetonitrile（95∶4∶1） and methanol-1% ice acetic（50∶50） as mobile phase,the flow rate were 1.0 mL·min-1 and 1.2 mL·min-1,respectively.Result：Under each condition,The calibration curves of catalpol and cinnamicacid were linear in the ranges of 3.36-168,0.156-104 mg·L-1,respectively.And the each average recoveries of the method were 101.59% for catalpol（RSD 1.36%,n=5）,101.28.% for cinnamicacid（RSD 1.28%,n=5）.Conclusion：The method is simple,accurate and repeatable,it can be used for quality control of Yanbianning granule.