HPLC同时测定藏药花锚中齐墩果酸和熊果酸含量

Determination of Oleanolic Acid and Ursolic Acid in Halenia elliptica by HPLC

目的:建立同时测定藏药花锚中齐墩果酸和熊果酸含量的测定方法。方法:采用高效液相色谱法,流动相甲醇-0.5%磷酸水(85:15),柱温20℃,流速0.8 mL.min-1,检测波长210 nm。结果:齐墩果酸和熊果酸在0.01～0.05,0.02～0.1 g.L-1呈良好的线性关系,加样回收率分别为101.17%,101.79%,RSD分别为1.90%,1.65%。结论:该法简便、快捷,结果准确、重复性好,可用于花锚药材中齐墩果酸和熊果酸的质量控制。

Objective：To establish a determination method of the content of oleanolic acid and ursolic acid in Halenia elliptica by HPLC.Method： The mobie phase was a mixture of methanol-0.5%H3PO4（85∶15）,the column temperature was set 20 ℃,the flow rate was 0.8 mL·min-1,the deterion wave leght was 210 nm.Result： The linear range of oleanolic acid was 0.01-0.05 g·L-1,the linear range of ursolic acid was 0.02-0.1 g·L-1,and the average recovery was 101.17% with RSD 1.90% and 101.79% with RSD 1.65%.Conclusion： The method was rapid,convenient,accurate,and suitable for quality control of oleanolic acid and ursolic in H.eliptica.