红树林底泥放线菌(N2010-37)发酵液的化学成分

Chemical Compounds of Actinomycete strain(N2010-37) of Bottom Mud in Mangrove

目的:研究红树林底泥放线菌(N2010-37)中化学成分。方法:放线菌(N2010-37)发酵菌丝体经95%乙醇提取,溶剂分级萃取,应用多种柱色谱分离和谱学分析方法对乙酸乙酯萃取部位化学成分进行研究。结果:从乙酸乙酯部位分离得到7个化合物,分别为6,7-二甲氧基-5,8-二羟基黄酮(Ⅰ),5,6,7,8-四甲氧基黄酮(Ⅱ),对羟基苯甲酸(Ⅲ),对羟基苯甲醛(Ⅳ),尿嘧啶(Ⅴ),腺嘌呤核苷(Ⅵ),胡萝卜苷(Ⅶ)。结论:化合物Ⅰ和化合物Ⅱ首次从海洋放线菌次级代谢产物中分离得到,化合物Ⅲ～Ⅶ为首次从该放线菌中分离得到。

Objective：To study the chemical components of the cultured filaments of an actinomycete strain（N2010-37）.Method： Compounds were isolated and purified by chromatographic techniques and recrystallization,and the structures were identified by spectral methods together with physiochemical analysis.Result： Seven compounds were identified.There were 6,7-dimethoxy-5,8-dihydroxylflavone（Ⅰ）,5,6,7,8-tetramethoxyflavone（Ⅱ）,p-hydroxybenzonic acid（Ⅲ）,p-hydroxybenzaldehyde（Ⅳ）,uracil（Ⅴ）,adenosine（Ⅵ）,daucosterol（Ⅶ）.Conclusion： Compounds Ⅰand Ⅱ were isolated from marine actinomycete for the first time.Compounds Ⅲ-Ⅶ have been isolated from this actinomycete for the first time.