淫羊藿苷对大鼠颈总动脉损伤后基质金属蛋白酶-2表达的影响

Effect of Icariin on the Expression of Matrix Metalloprotease-2 after Vascular Injury in Rats

目的:研究淫羊藿苷对大鼠颈动脉损伤后基质金属蛋白酶(MMP-2)mRNA表达的影响。方法:取大鼠44只,随机分为正常对照组8只,淫羊藿苷高、低剂量组和模型组各12只。后3组建立鼠颈动脉球囊损伤术后狭窄模型,淫羊藿苷组术前6 d开始ig淫羊藿苷60,120 mg.kg-1至实验结束(14 d和28 d);模型对照组以等体积生理盐水ig。术后14 d和28 d分批处死动物。取各组实验动物颈动脉损伤段,常规制片,免疫组织化学S-ABC法检测MMP-2蛋白的表达;RT-PCR检测MMP-2 mRNA的表达。结果:在球囊损伤后14,28 d时,淫羊藿苷干预组的MMP-2 mRNA及蛋白表达量显著低于模型组(P<0.01)。各干预组之间无明显差异。结论:淫羊藿苷在动脉损伤后早期可通过下调MMP-2 mRNA的表达,抑制平滑肌细胞的迁移,从而减轻细胞外基质(ECM)的合成而抑制球囊损伤后新生内膜的增生。

Objective：To observe the effect of icariin（ICA） on the levels of matrix metalloprotease-2（MMP-2） in rat carotid artery injured by balloon and to evaluate the mechanisms of inhibiting intima hyperplasia and vascular remodeling.Method： Fourty-four rats were randomly divided into 4 groups： natural group,model group and ICA low dose group（60 mg·kg-1） and ICA high dose group（120 mg·kg-1）.Rat carotid balloon injury restenosis model was established.Rats of ICA group were treated with（ig） drugs for 2 or 4 weeks.Then the mRNA expression of MMP-2 was assessed by RT-PCR,and immunohistochemistry analysis was applied to detect the expression of MMP-2.Result： Compared with model group,the ICA group significantly decreased the expression of MMP-2,at 14 and 28 day after injury（P0.01）.Conclusion： ICA could inhibit neointimal proliferation of rat carotid artery injured after balloon injury,the mechanism might be related to the effects of ICA in decreasing mRNA level of MMP-2,inhibiting migration of vascular smooth muscle cells.