2型糖尿病大鼠视网膜病变模型的建立及羟苯磺酸钙的干预作用

Rat Models of Type 2 Diabetic Retinopathy and the Intervention of Calcium Dobesilate on the Model

目的:建立及评价2型糖尿病大鼠视网膜病变(DR)模型,观察羟苯磺酸钙对DR的干预作用。方法:72只SD大鼠随机分成正常组和造模组。造模组大鼠以高脂饲料喂养3个月后ip STZ(45 mg.kg-1)1次。12周后造模大鼠再随机分为模型组和药物组。药物组每日ig羟苯磺酸钙1次(150 mg.kg-1),给药周期2个月结束后,检测视网膜组织中脂质过氧化物丙二醛(MDA),超氧化物歧化酶(SOD),谷胱甘肽(GSH)含量,血管内皮生长因子(VEGF),糖基化产物(AGEs)等指标。结果:与正常组比较,模型组各指标均有明显改变;与模型组比较,羟苯磺酸钙组SOD的活力,VEGF的表达有显著性差异(P<0.05),但GSH,MDA含量及AGEs的表达差异不明显。结论:本实验2型糖尿病大鼠视网膜病变模型与人类2型糖尿病视网膜病变有很多共性,且能被羟苯磺酸钙所干预,可能为研究2型糖尿病视网膜病变及其防治药物的理想动物模型之一。

Objective：Establishment and evaluation of type 2 diabetic retinopathy model in rats,exploring the efffects of calcium dobesilate on the model.Method： SD rats were randomly divided into normal group and model group,model rats with high fat diet for 3 months,then ip STZ（45 mg·kg-1） one times.12 weeks later,the model rats were randomly divided into model group and drug group.Drug group ig daily calcium dobesilate（150 mg·kg-1）.After the administration period,detection of retinal tissue malondialdehyde（MDA）,superoxide dismutase（SOD）,glutathione（GSH）,vascular endothelial growth factor（VEGF）,glycosylated of products（AGEs） and other indicators.Result： Compared with model group,drug group SOD activity,VEGF expression are significantly different（P 0.05）,but GSH,MDA content and the expression of AGEs was not significantly different.Changes of the aboved index proved that the model was successful.Conclusion： The experimental rat model was established successfully.It may provide a perfect animal model for investigating type 2 diabetic retinopathy and pharmacology successful.