系统性红斑狼疮患者单个核细胞(PBMC)中ITGAL的表达及意义

目的探讨ITGAL基因在系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中的表达及其意义。方法分离SLE患者及正常人群PBMC,采用Qiagen RNA/DNA mini kit提取RNA,实时-定量PCR检测ITGAL mRNA的表达,对SLE患者的ITGAL表达与红斑狼疮疾病活动指数(采用SLEDAI评分)进行相关分析。结果活动性SLE患者中ITGAL mRNA表达较正常对照组明显升高,两组比较有统计学意义(P<0.001),与SLEDAI评分呈正相关(r=0.376,P<0.05)。结论活动性SLE患者PBMC的ITGAL mRNA表达明显上升,其变化与疾病活动程度呈正相关,ITGAL表达可能与SLE发病有关。

Objective To investigate the significance of ITGAL mRNA expression in PBMC of lupus patients. Methods Ten out of 20 SLE patients had active disease and 10 patients with lower disease activity were recruited, PBMC were isolated by Ficoll-Hypaque density gradient centrifugation, the mRNA levels of ITGAL genes were measured by real-time quantitative polymerase chain reaction. Results ITGAL mRNA levels were significantly increased in active lupus PBMC compared with healthy controls（P〈0.001） The mRNA levels of ITGAL genes were positively correlated with increased disease activity in lupus patients as measured by SLEDAI （r=0.376, P〈0.05）. Conclusion The expression of ITGAL in PBMC of lupus is higher than that in healthy controls, its change is related with disease activity in lupus patients, which suggests that ITGAL could be involved in the pathegenesis of SLE.