摘要
目的研究胃癌患者的癌组织线粒体DNA(mtDNA)D-loop区碱基突变情况,以探讨mtDNA D-loop区碱基突变与胃癌发生发展的相关性。方法采用聚合酶链反应和DNA测序相结合的方法,对75例胃癌患者癌组织线粒体D-loop区进行扩增并测序分析。结果在75例胃癌组织中共有130个位点发生了变化,其中属于基因多态性有101个,基因突变有29个。75例胃癌组织中39例mtDNA D-loop区存在突变,突变率为52%。结论 mtDNA D-loop区碱基突变可能在胃癌发生发展中发挥重要作用。
Objective To investigate whether the mtDNA D-loop mutation plays a role in gastric cancer. Methods The tumor tissues from 75 gastric cancer patients were analyzed by PCR and DNA sequencing, with special attempt to characterize the spectrum of mtDNA D-loop somatic mutations and their potential significance in. cancer diagnosis and development were evaluated. Results Among the 75 cases, 29 mutations and 101 polymorphisms were identified. And the mutations rate is 52%（39/75）. Conclusion Based on the mtDNA mutation pattern in tissues of gastric cancer observed in this study, it concluded that mtDNA D-loop mutations play a role in gastric cancer.
出处
《当代医学》
2011年第34期34-36,共3页
Contemporary Medicine
