玫瑰糠疹患者单一核细胞转录因子T-betmRNA及GATA-3mRNA的表达

Expressions of T-bet and GATA-3 mRNA in peripheral blood mononuclear cells of patients withpityriasis rosea

目的探讨T-bet、GATA-3在玫瑰糠疹发病机制中的意义。方法SYBRGreenI实时荧光定量PCR技术检测17例玫瑰糠疹患者和20例正常人外周血单一核细胞中T-betmRNA和GATA-3mRNA的表达。结果玫瑰糠疹患者外周血中T-betmRNA与GATA-3mRNAAvg△Ct值分别为3．33-t-O．94与5．22±0．69，对照组分别为4．31±1．11与4．36±1．02。患者的T-betmRNA表达高于对照组（P〈0．05），GATA-3表达低于对照组（P〈0．05）。结论玫瑰糠疹患者外周血中T-bet／GATA-3升高，Thl优势表达，细胞免疫异常在玫瑰糠疹发病过程中可能具有重要作用。

Objective -To investigate the significance of T-bet and GATA-3 in the pathogenesis of pityriasis rosea. Methods SYBR Green I real-time fluorescence quantitative PCR was performed to detect the expressions of T-bet and GATA-3 mRNA in peripheral blood mononuclear cells （PBMCs） from 17 patients with pityriasis rosea and 20 normal human controls. Results The average delta Ct value of T-bet mRNA and GATA-3 mRNA was 3.33 ＋ 0.94 and 5.22 ＋ 0.69 respectively in the patients, 4.31 ＋ 1.11 and 4.36 ± 1.02 respectively in the controls. There was a higher expression of T-bet mRNA but a lower expression of GATA-3 mRNA in the patients compared with the controls （both P 〈 0.05）. Conclusions Patients with pityriasis rosea show an increased ratio of T-bet to GATA3 expression and predominant expression of Thl-type cytokines. Abnormal cellular immunity may play an important role in the pathogenesis of pityriasis rosea.