尖孢镰刀菌与草甘膦复配对空心莲子草防御酶的影响

Influence of Fusarium oxysporum mixed with Glyphosate on Alternanthera philoxeroides defense enzymes

从自然发病的空心莲子草中分离到尖孢镰刀菌，室内抑菌试验表明，尖孢镰刀菌对空心莲子草有较强的抑制作用，接种9d后．病情指数为46．69。尖孢镰刀菌与草甘膦以1：1、3：1、9：1混合，喷洒至健康空心莲子草上，测定过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性。结果表明：POD活性在48—96h随着时间的延长而上升，1：1复配剂96h后出现高峰，3：1复配剂48h后达到高峰，9：1复配剂72h达到高峰。9：1复配剂48h后SOD活性出现最高峰，较对照提高了40．0％。9：1复配剂48h内CAT出现了活性高峰．随后急剧下降。

Fusarium oxysporum isolated from Alternanthera had strong inhibited effect on Alternanthera with disease index of 46.69 in 9d indoor bacteria-inhibiting test. Leaf peroxidase （POD）, superoxide dismutase （SOD）, and catalase （CAT） activity were measured after spraying Fusarium oxysporum suspension mixing with glyphosate of 1：1, 3：1, and 9：1 ratios, on healthy A. philoxeroides plants. It was shown that, POD activityincreased by time with highest activity in 1：1 mix after 96 h spraying, while in 3：1 mix after 48 h, and in 9：1 mix after 72 h. Highest SOD activity was observed after 48 h in 9：1 mix spraying, together with highest CAT activity after 48 h spraying, and then decreased sharply.