摘要
目的:观察大鼠胶质细胞是否参与了D-半乳糖诱导的脑衰老的过程。方法:采用D-半乳糖制备动物大鼠衰老模型,生化分光光度法检测大鼠脑海马氧化应激水平,免疫组化方法观察大鼠海马星形胶质细胞和小胶质细胞的表达和形态变化。结果:大鼠模型组较对照组海马氧化应激水平增高,星形胶质细胞和小胶质细胞表达增多,染色增强。免疫荧光染色结果显示,模型组胶质细胞和诱导型一氧化氮合酶有共存关系,模型组海马一氧化氮水平升高。结论:大鼠胶质细胞可能参与了D-半乳糖诱导脑衰老的过程。
Objective： To investigate the D-gal-induced brain aging process in rat glial cells.Methods： Aging rat animal model was prepared by D-galactose,and oxidative stress levels were tested by biochemical spectrophotometry in rat hippocampus.Expression and morphological changes of hippocampal astrocytes and microglia in rat were observed by immunohistochemistry.Results： In the hippocampus,the levels of oxidative stress were increased in rat model group than that the control group,the expressions of astrocytes and microglial cells had increased and staining enhancement.Immunofluorescence staining showed that glial cells in the model group were co-localization with inducible nitric oxide synthase,and the hippocampal nitric oxide levels were increased in the model group.Conclusion： Rat glial cells may be involved in D-gal-induced brain aging process.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2011年第6期675-680,共6页
Chinese Journal of Neuroanatomy
基金
江苏省教育厅2010年大学生实践创新训练计划(806)
江苏建康职业学院科技发展基金(JK201004)
