微囊化异种嗅鞘细胞移植联合中药修复大鼠脊髓损伤

Study on repair of spinal cord injury of rats by heterotransplantation of microencapsulated ensheathing cells combined with China herb

目的:旨在探讨基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在异种嗅鞘细胞移植联合中药干预下脊髓损伤中的作用。方法:将188只SD大鼠随机分为正常对照组、单纯损伤组、异种嗅鞘细胞移植组、异种嗅鞘细胞移植联合中药治疗组,损伤组又设1、3、7、14、28 d 5个时相点,免疫组织化学技术检测MMP-9的表达,同时电镜观察血脊髓屏障的变化。结果:脊髓损伤后血脊髓屏障的超微结构不同程度损害、MMP-9表达上调,经干预治疗后出现一定程度的恢复,MMP-9表达下调。结论:脊髓损伤后MMP-9表达水平增加与伤后血脊髓屏障损害的发生、发展密切相关,MMP-9在脊髓损伤后血脊髓屏障损害的病理生理过程中起重要作用。异种嗅鞘细胞移植联合中药对脊髓损伤有较好的修复作用。

Objective： To explore the function of（matrix metalloproteinase-9,MMP-9） in heterotransplantation of olfactory ensheathing cells combined with China herb intervention after spinal cord injury.Methods： Healthy 188 SD rats were divided into three groups as following： normal,injury control,transplantation of olfactory ensheathing cells,transplantation of olfactory ensheathing cells combined with China herb.Posttraumatic tissues were achieved at 1,3,7,14 and 28 d after spinal cord injury.The expression of MMP-9 with immunohistochemistry were measured in three groups respectively.Electron microscope was used to observe the changes in blood-spinal cord barrier.Results： Blood-spinal cord barrier was damaged in some extent recoveried;the expression of MMP-9 was obviously upregulated after spinal cord injury.The ultrastructure of blood-spinal barrier was partly recoveried and the expression of MMP-9 was decreased after intervention.Conclusion： Increase of the expressional levels of MMP-9 has a closely correlation with production and development of blood-spinal cord barrier damage after cervical spinal cord trauma.MMP-9 plays important roles in the pathophysiological process of blood-spinal cord barrier damage after cervical spinal cord trauma.Heterotransplantation of olfactory ensheating cells combined with China herb is effective in the repair of spinal cord injury.