芹菜素的急性毒性、遗传毒性及亚慢性毒性试验研究

Acute toxicity,genetic toxicity and sub-chronic toxicity of apigenin

目的研究芹菜素的安全性,为其合理开发利用提供科学依据。方法根据《食品安全性毒理学评价程序和方法》,进行了芹菜素急性毒性试验、小鼠骨髓微核实验、Ames试验、小鼠精子畸变试验、大鼠90天喂养试验。结果芹菜素大鼠急性毒性的MTD大于8 g/kg BW,遗传毒性试验结果为阴性,未见芹菜素对大鼠体重、摄食量、食物利用率、血常规、血生化、尿常规有生物学意义的影响。芹菜素对大鼠脏器无明显影响。结论芹菜素属实际无毒物,无遗传毒性,芹菜素的大鼠90天喂养实验的NOAEL为8 g/kg BW。

Objective To evaluate the safety of apigenin,so as to provide scientific support for the reasonable use and development of apigenin.Methods According to the procedure and methods in the Food Toxicological Safety Evaluation,a series of toxicological studies on the ingestion of apigenin were conducted,including acute toxicity,genetic toxicity as well as sub-chronic toxicity experiments.Results For the acute toxicity,the maximum tolerated dose（MTD） was higher than 8 g/kg BW.No apigenin-related genetic toxicity was observed.There were no apigenin-related adverse effects on body weight,food intake,food consumption,hematology,serum biochemistry,urine routine as well as histopathology in rats.Conclusion The no observed adverse effect level（NOAEL） of apigenin was 8 g/kg BW.Apigenin belongs to actually non-toxic substance with no genetic toxicity.