云南黑籽南瓜种质遗传多样性的RAPD和ISSR分析

Genetic Diversity of Cucurbita ficifolia Bauche Based on RAPD and ISSR

采用RAPD和ISSR分子标记技术对来源于云南省6个地州13份黑籽南瓜种质进行遗传多样性分析。结果表明:6个RAPD和6个ISSR引物分别扩增出43条和41条带,多态性比率分别为90.70%和51.21%;RAPD和ISSR标记检测供试材料的遗传相似性系数范围分别为0.340～0.895和0.162～0.941I,SSR检测多态性效果高于RAPD。RAPD标记聚类分析将供试种质分为3个类群5组;ISSR标记聚类分析将供试种质分为4个类群6组,RAPD和ISSR标记的遗传相似性系数呈显著相关。基于UPGMA聚类结果,可为黑籽南瓜的引种栽培或品种改良提供参考。

Random amplified polymorphic DNA（RAPD）and inter-simple sequence repeat（ISSR）molecular markers were used to detect the genetic diversity among 13 Cucurbita ficifolia resources from 6 prefecture of Yunnan.The results showed that 43 and 41 bands were obtained by RAPD and ISSR markers amplified through 6 selected primers respectively.The PPB（percentage of polymorphic bands）in RAPD detection（90.70%）was higher than that in ISSR（51.21%）.The similarity coeficient ranging from 0.340 to 0.895 and from 0.162 to 0.941 respectively,and the ISSR was more efficient than RAPD.The germplasms were divided into three main cluster groups and five inferior groups by RAPD,and it also could be divided into four main cluster groups and six inferior groups by ISSR.The significant correlation between RAPD and ISSR markers was observed.The dendrogram was constructed by UPGMA method could provide theoretical basis for Cucurbita ficifolia introduction culture or cross breeding in future.