百合远缘杂交育种及其真实性鉴定

Wide Cross Breeding of Lily and Identification of Authenticity

以5种野生百合与2个杂种系百合品种为亲本,设计6个杂交组合进行远缘杂交,通过子房与田间培养成功获得远缘杂交种并采用形态学与RAPD分子标记对其真实性进行综合鉴定。结果显示,杂交组合麝香百合×条叶百合通过子房培养获得杂交苗3株;条叶百合×王百合、条叶百合×雪皇后、细叶百合(黑龙江种源)×雪皇后、细叶百合(黑龙江种源)×西伯利亚与细叶百合(承德种源)×西伯利亚各杂交组合均获杂交种子,结子率分别为27.0%1、.4%、31.5%、0.04%与0.08%。杂交种株型在苗期表现为偏母植株与中间型植株,其他形态学指标介于亲本之间或优于亲本;RAPD分子标记鉴定表明,杂种植株既扩增出相加性带型、单亲本带型,又扩增出融合双亲的特异性带型。获得杂交种为真杂种可用于百合品质育种。

Using five wild lily and two lily cultivars as parents.Designing six cross-combination for wide cross breeding.Distant hybrids was obtained successfully by field planting way and ovary culture finally.They were identified by morphology and RAPD molecular identification.The results showed that crossing combination Lilium longiflorum×Lilium callosum obtaining three hybrid seedlings by ovary culture,Lilium callosum×Lilium regale,Lilium callosum×＇Snow Queen＇,Lilium pumilum（Provenance of Heilongjiang）×＇Snow Queen＇,Lilium pumilum（provenance of Heilongjiang）×＇Siberia＇,Lilium pumilum（provenance of Chengde）×＇Siberia＇ obtaining hybrid seeds.The seed set ratio were 27.0%,1.4%,31.4%,0.04% and 0.08% respectively.Plant type of hybrid seedlings are matroclinous plants and intermediate plants.The other indexs of morphology lies between parents or better than parents.RAPD molecular identification showed that the hybrids has not only additive banding,solo-parent banding,but also extraordinary banding which is the fusion of its parents,and new bandings appearing in the patterns.The hybrids are true hybrid and can be used to quality breeding of Lily.