IL-17R在韦格纳肉芽肿患者外周血免疫细胞的表达

IL-17R expression on immune cells in peripheral blood of Wegener′s granulomatosis patients

目的检测韦格纳肉芽肿(WG)患者外周血免疫细胞特别是B淋巴细胞和浆细胞上IL-17受体(IL-17R)的表达有无异常。方法流式细胞仪检测正常人(HC)及WG患者外周血免疫细胞表面IL-17R蛋白的表达。结果 IL-17R在WG患者外周血中性粒细胞(PMN)、单核细胞(Mono)、总淋巴细胞(Lym)上的表达与正常人无显著差异,但在B淋巴细胞(CD19+CD20+)(P<0.05)和浆细胞(CD19+CD38+)(P<0.05)上的表达显著高于正常人;活动期与非活动期患者、环磷酰胺(Cyc)和甲氨蝶呤(MTX)治疗组患者IL-17R的表达无显著差异。结论 IL-17R在WG患者外周血B淋巴细胞和浆细胞上的表达显著升高,但在中性粒细胞和单核细胞上的表达无明显异常。

Objective To assess whether the receptor of IL 17 （IL- 17R） expression on peripheral blood immune cells including B lymphocytes and plasma cells of Wegener＇s granulomatosis （WG） patients is abnormal. Methods Flow cytometry was used to analyze the expression of IL- 17R protein on peripheral blood immune cells of health control （HC） and WG patients. Results There was no difference of IL- 17R expression on PMN, monocytes, total lymphocytes between WG patients and HC. However, IL- 17R expression on B lymphocytes （P = 0. 039） and plasma cells （ P = 0. 043） were much higher in WG patients compared with HC. There was no difference of IL- 17R expression on these cells between active and inactive WG, cyclophosphamide （Cyc） and methotrexate （MTX） treated WG. Conclusion IL- 17R expression on B lymphocytes and plasma cells are significantly increased in WG patients, but normal on PMN and monocytes.