弓形虫感染对雌鼠性器官凋亡相关蛋白Bax和Bcl-2表达的影响

Effect of Toxoplasma gondii infection on the female sex organ apoptosis-related proteins Bax and Bcl-2 in mice

目的采用弓形虫感染雌性小鼠，观察弓形虫对卵巢、输卵管、子宫、阴道等性器官组织细胞中凋亡相关蛋白Bax、Bcl-2表达的影响，分析和探讨凋亡调控蛋白bax和bcl-2的表达是否与生殖毒性有关。方法10周龄的处女鼠18只，随机分为感染组和正常对照组，9只／组。感染组腹腔注射纯化弓形虫速殖子0．2ml（160个／只），正常组腹腔注射等量PBS（0．01mol／L；pH7．4）。观察、记录小鼠的饮食及存活等状况；于感染后第21天处死小鼠，取卵巢、输卵管、子宫、阴道组织固定、脱水、包埋、切片，用苏木精一伊红染色法染色，观察各组织的病理变化；免疫组化检测各组织中细胞凋亡相关蛋白Bax、Bcl-2的表达。结果正常组小鼠无异常表现，染虫组小鼠食量少、毛疏松、竖立。弓形虫速殖子主要分布于小鼠的卵巢、输卵管及子宫的间质。感染后卵巢、输卵管、子宫内膜和阴道黏膜充血、水肿，炎性细胞浸润；宫腔部分闭锁，阴道黏膜皱壁减少。卵巢、输卵管、子宫、阴道各组织细胞凋亡相关蛋白均有表达，Bax的表达量随着染虫数的增加而增加，而Bcl-2的表达量是随着染虫量的增多而减少，与正常组相比差异均有统计学意义（P〈0．01）。结论弓形虫感染小鼠，可侵人性器官，并引2起各组织结构发生病理学变化，随着染虫量的增多，可导致细胞凋亡相关蛋白的表达异常。

Objective To explore and analyze the relation between the expression of the apoptosisrelated protein bax and bel-2 in the sexual organs, female mice were infected with Toxoplasma gondii and it was observed that the expression of the apoptosis-related protein bax and bcl-2 in the sexual organs （ovaries, fallopian tubes, uterus, vagina）. Methods Eighteen 10-week-old virgin mice were randomly divided into two groups： infected group and control group. Mice in the infected group were injected purified Toxoplasma gondii taehyzoites （ 160 tachyzoites/0.2 ml ） , and control group were injected equivalent PBS （ 0.01 tool/L, pH 7.4）. Mice of infected with T. gondii were dynamically observed through the survival rate and situation of food changes. Mice were killed on the 21st day after infection,and the ovaries, fallopian tubes, uterus, and vaginal tissue were collected. Then theose tissues were fixed,dehydrated, embedded, cut into slices, and stained to observe the pathological changes and the expression of apoptosis-related proteins Bax, Bcl-2 with hematoxylin- eosin staining（HE） and Immunohistochemical method. Results Mice in the infected group had hair loose, erect and less appetite. The organizations of ovaries, fallopian tubes, uterus and vagina in the infected group Toxoplasma gondii was found. The ovarian, tubes, uterus and vaginal were slightly swollen, enlargement, hyperemia, edema, and with many inflammatory ceils. Part of Utreine was locked and mucous membrane and folds reduced, but those hsd no abnormalities in the control group. Simultaneously the expresseion of apoptosis- related proteins Bax increased and Bcl-2 decreased in those organizations of the infected group with the increasing days of Toxoplasma gondii infection. Both changes were significant in the infected group, compared with the control group （ P 〈 0. 05 ）. Conclusion Toxoplasma gondii could invade the sexual organs, and caused pathological changes in the organizational structure while infecting mice. And that could lead to the expression of apoptosis-related proteins abnormally along with the increasing days of Toxoplasma gondii infecting.