黄体酮前体脂质体口服制剂水合后包封率的测定

Determination of the entrapment efficiency of oral progesterone proliposome preparation

目的:为了提高口服黄体酮后的生物利用度,开发黄体酮口服制剂,制备了一种新型黄体酮前体脂质体口服制剂,并测定了其水合后的包封率。方法:采用一种新型前体脂质体制备方法将黄体酮制成前体脂质体,开发新型前体脂质体口服制剂。采用葡聚糖Sephadex G-50凝胶柱对黄体酮脂质体进行柱分离,测定其包封率。结果:包封率与稀释倍数和药脂比有关。当药脂比为1∶20、稀释倍数为1∶10时,黄体酮前体脂质体包封率可达(72.36±11.69)%。结论:利用前体脂质体制备技术可将黄体酮包裹成脂质体,所形成的脂质体包封率较高,可为黄体酮前体脂质体口服制剂的开发奠定基础。

Objective： To improve the oral bioavailability of progesterone,a new kind of oral proliposome was prepared,and the entrapment efficiency of progesterone proliposome was determined.Methods： Progesterone liposome solution was separated and purified through a Sephadex G-50 column.Results： The entrapment efficiency varied with different ratios of drug/phospholipid and dilution times.The entrapment efficiency of the progesterone liposome was（72.36±11.69）% when the drug/phospholipid was 1∶20 and the dilution time was 1∶10.Conclusion： This analytical method for determining the entrapment efficiency is reliable and the entrapment efficiency of obtained progesterone liposome is high.This will provide a reference for study of oral progesterone proliposome preparations.