一株南极大磷虾共附生细菌的分离鉴定及一氧化氮靶向抑制活性筛选

Isolation,identification and screening of nitric oxide targeted inhibition bioactivity of a symbiotic/epiphytic bacterium from Euphausia superba

从我国南极磷虾科学探捕项目所采集的南极大磷虾(Euphausia superba)样品中分离纯化到一株共附生细菌,对其进行了细菌多相分类学分析、发酵培养、代谢产物提取及HPLC分析,并对其发酵粗提物进行了针对多种重大疾病药物靶标-一氧化氮(NO)抑制活性的靶向筛选。结果表明,该菌株与其进化关系最近的需盐杆菌(Salegentibacter salinus)ISL-6之间的16S rRNA基因序列同源性为93.2%(小于确定新种的阈值97%)。综合该菌株的多相分类学指标分析结果,将其鉴定为需盐杆菌属的一个新种,命名为Salegentibacter sp.NJC-06,其发酵代谢产物中主要活性成分为富含双键的萜类物质,发酵液粗提物具有较强的NO靶向抑制活性。

A new symbiotic/epiphytic bacteria,NJC-06 strain,was isolated from Euphausia superba samples captured at FAO 48.1 area in Antarctica Ocean.The polyphase taxonomic identification was then performed.HPLC analysis was done after microbial strain fermentation and culture extraction.The screening of nitric oxide（NO） targeted bioactivity of the crude extract was subsequently carried out.The polyphase identification analysis showed that the strain belonged to the genus of salegentibacter.The strain was most close to Salegentibacter salinus ISL-6 strain with the similarity value of 93.2%（less than the threshold value of 97% for the new specie establishment） based on 16S rRNA gene sequence homology analysis.According to these integrated analysis,this strain was finally identified as a new strain and nominated as Salegentibacter sp.NJC-06.The HPLC analysis of the culture extract demonstrated that the main compound in the crude extract belonged to terpene-like substance and the crude extract showed significant inhibitory bioactivity against NO.