皮肌炎/多肌炎患者血清对血管内皮细胞的影响

Effects of sera from patient with dermatomyositis/polymyositis on human umbilical vein endothelial cells in vitro

目的:探讨皮肌炎/多肌炎(dermatomyositis/polymyositis,DM/PM)患者血清对血管内皮细胞活性的影响。方法:取16例未经治疗的DM/PM患者血清为DM/PM组,其中8例治疗前取血清作为治疗前组,经治疗病情好转后再次取血清作为治疗后组,13名健康体检者血清为正常组。体外培养脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC),MTT法检测血清对HUVEC活性的影响。结果:DM/PM组与正常组血清对HUVEC活性的影响差异均无统计学意义(P>0.05)。治疗后组血清与正常组相比,在24 h时对HUVEC活性呈抑制作用(P<0.01),而在72 h则表现为增强作用(P<0.05)。治疗后血清对HUVEC活性的影响在24 h时低于治疗前(P<0.05),而在48 h和72 h差异均无统计学意义(P>0.05)。结论:DM/PM患者血清中可能不存在影响血管内皮细胞活性并直接导致其损伤的因素,治疗后的血清体外对HUVEC活性的影响是先抑制后促进。

Objective：To investigate the influence of serum from patients with dermatomyositis/polymyositis（DM/PM） on the activity of human umbilical vein endothelial cells（HUVEC）.Methods：The serum taken from 16 untreated DM/PM patients were enrolled in the DM/PM group who were evenly divided into pre-treatment group（8 cases） and post-treatment group（8 cases）,and the serum from 13 healthy people acted as control.The HUVEC was cultured in vitro,and the influence of serum from patients with DM/PM on HUVEC was examined by MTT assay.Results：There was no significant difference in the activity of HUVEC between the DM/PM group and the control group（P0.05）.Compared with the control,the serum from the post-treatment group suppressed the proliferation of HUVEC at 24 h after treatment（P0.01）,but accelerated the proliferation of HUVEC at 72 h（P0.05）.Compared with the pre-treatment group,the serum from the post-treatment group suppressed the proliferation of HUVEC at 24 h after treatment（P0.05）,but no significant difference was observed between the two groups at 48 h and 72 h（P0.05）.Conclusions：The serum from patients with DM/PM may not affect or impair the activity of HUVEC in vitro;the serum from the post-treatment DM/PM patients may inhibit the activity of HUVEC after the first promotion in vitro.