向日葵胰蛋白酶抑制剂SFTI的合成及酶抑制动力学研究

Synthesis and enzyme inhibition kinetic study of sunflower seed trypsin inhibitor

目的考察合成的向日葵胰蛋白酶抑制剂的抑制效果,计算其抑制常数Ki。方法采用Fmoc固相化学合成方法,反相柱纯化后用CytofluorTM微孔板发光检测仪测定向日葵胰蛋白酶抑制剂的抑制常数。结果与结论向日葵胰蛋白酶抑制剂的Ki值为2.2nM±0.5(n=8),抑制效果良好。

OBJECTIVE To investigate the inhibitory constant of synthesised sunflower seed trypsin inhibitor.METHODS Using fmoc solid phase peptide synthesis and reverse phase column for purification,after that using cytofluortm microtitre plate flurometer to test inhibitory constant of synthesised sunflower seed trypsin inhibitor.RESULTS and CONCLUSION The inhigitory constant of synthesised sunflower seed trypsin inhibitor is 2.2nM±0.5（n=8）,having good inhibitory effect.