反相高效液相色谱法同时测定桂油生物催化反应中肉桂醇、苯甲醛、苯乙酮、肉桂酸和肉桂醛

Determination of cinnamyl alcohol,benaldehyde,cetophenone,cinnamic acid and cinnamaldehyde in cinnamon biocatalysis reaction by RP-HPLC

建立了快速测定桂油生物催化反应中肉桂醇、苯甲醛、苯乙酮、肉桂酸和肉桂醛的反相高效液相色谱方法。色谱条件:采用Kromasil-100A C18柱(250 mm×4.6 mm,5μm),以乙腈-甲醇-水-冰醋酸(体积比36:6:58:0.02)为流动相,流速为1.0 mL/min,检测波长为205 nm,柱温为室温。结果表明,肉桂醇在1.03～82.16μg/mL范围内呈良好线性关系(r=0.999 9);苯甲醛在1.10～87.92μg/mL范围内呈良好线性关系(r=0.999 9);苯乙酮在1.06～84.48μg/mL范围内呈良好线性关系(r=0.999 8);肉桂酸在1.30～104.16μg/mL范围内呈良好线性关系(r=0.999 8);肉桂醛在1.21～96.48μg/mL范围内呈良好线性关系(r=0.999 2)。肉桂醇、苯甲醛、苯乙酮、肉桂酸和肉桂醛的平均回收率分别为99.8%,100.7%,100.6%,96.4%和100.7%,相对标准偏差(RSD)分别为3.4%,3.3%,2.0%,1.8%和5.5%。

The method for determination of cinnamyl alcohol,benaldehyde,acetophenone,cinnamic acid and cinnamaldehyde in cinnamon biocatalysis reaction was established.The determination by RP-HPLC was carried out on a Kromasil-100A C18 column（250 mm×4.6 mm,5 μm）at room temperature,with acetonitrile-methanol-water-glacial acetic acid（36∶ 6∶ 58∶ 0.02）as mobile phase at 1.0 mL/min,and the detection wavelength was 205 nm.The linear range of cinnamyl alcohol is 1.03~82.16 μg/mL（r=0.999 9）;the linear range of benaldehyde is 1.10~87.92 μg/mL（r=0.999 9）;the linear range of acetophenone is 1.06~84.48 μg/mL（r=0.999 8）;the linear range of cinnamic acid is 1.30~104.16 μg/mL（r=0.999 8）;the linear range of cinnamaldehyde is 1.21~96.48 μg/mL（r=0.999 2）.Average recovery of cinnamyl alcohol,benaldehyde,acetophenone,cinnamic acid and cinnamaldehyde were 99.8%,100.7%,100.6%,96.4% and 100.7%,respectively,with standard deviation of 3.4%,3.3%,2.0%,1.8% and 5.5%,respectively.