摘要
采用反相高效液相色谱法测定湿生扁蕾中有效成分当药醇苷的含量.色谱条件Kromasil-C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇和水(含0.04%H3PO4)55∶45等度洗脱,流速0.8ml/min,检测波长254nm,柱温30℃.当药醇苷与其它组分达到基线分离,当药醇苷的线性范围为0.275-1.65μg(r=0.9997),加标回收率为99.6%(RSD=2.7%).该方法测定快速,结果准确,可靠.
To establish a quantitative method determination of swertianolin in Gentianopsis paludosa（Munro） Ma by RP-HPLC.The sample were separated on the column of Kromasil-C18（4.6mm×250mm,5μm） which eluted with 55% methanol and water（containing 0.04% phosphoric acid） with detected wavelength at 254 nm.The flow rate was 0.8 mL·min-1,and the column temperature was 30 ℃.The components were base-isolated.The linear ranges of swertianolin was 0.275-1.65μg（r=0.9997）.The average recovery rate was 99.6%（RSD=2.7%）.The method is rapid and precise.
