细胞周期蛋白在喉癌组织中的表达及意义

Expression and Significance of Cyclins in Laryngeal Carcinomas

目的探讨喉癌组织中细胞周期蛋白(Cyclin D1、Cyclin A、Cyclin B1、Cyclin E)的表达规律及其与喉癌临床病理参数的关系。方法采用免疫组化技术,检测Cyclin D1、Cyclin A、Cyclin B1、Cyclin E在67例喉癌组织和32例癌旁正常组织中的表达情况。结果 Cyclin D1、Cyclin A、Cyclin B1、Cyclin E在癌旁正常组织中呈低表达。随着喉癌分化程度的降低,Cyclin D1、Cyclin A、Cyclin B1、Cyclin E表达阳性率明显升高,高分化者、中分化者与低分化者比较有显著性差异(P<0.05)。结论通过免疫组织化学法分析细胞周期的数据,有利于肿瘤分级,且细胞周期数据与预后相关。

Objective To investigate the expression and significance of Cyclin D1,Cyclin A,Cyclin B1 and Cyclin E in laryngeal carcinomas,and study the correlation between parameters and clinicopathological features.Methods Expression of Cyclin D1,Cyclin A,Cyclin B1 and Cyclin E were analyzed by immunohistochemistry staining in 67 cases of laryngeal carcinomas and 32 peri-cancer normal larynx tissues.Results Cyclin D1,Cyclin A,Cyclin B1 and Cyclin E were low Expressed in peri-cancer normal larynx tissues.With the decreasing of pathological classification,the expression of Cyclin D1、Cyclin A、Cyclin B1 and Cyclin E in laryngeal carcinomas increased accordingly.The difference between group highly-differentiated、moderately-differentiated and poorly-differentiated were significant（P0.05）.Conclusion Cell cycle phase can be analyzed by immunohistochemical stain,and it is beneficial for tumor classification and helpful to predict the prognosis.