VEGF、bFGF血清浓度变化与骨肉瘤预后的关系探讨

The correlation between the level of serum VEGF and bFGF with metastasis and prognosis of osteosarcoma

目的:探讨血清VEGF、bFGF浓度在评价骨肉瘤患者的预后及其肿瘤转移复发中的价值。方法:采用双抗体夹心ELISA法检测52例骨肉瘤患者术前、术后和60例健康体检者血清血管内皮生成因子(VEGF)、碱性成纤维因子(bF-GF)表达水平,并分析其与骨肉瘤患者术后肿瘤转移复发的相关性。结果:52例骨肉瘤患者术前血清VEGF、bFGF表达水平明显高于健康体检者(P<0.01),术后血清VEGF、bFGF表达水平与术前相比均明显下降(P<0.01),但仍高于健康体检者(P<0.01)。术前血清VEGF、bFGF的表达水平分别随原发肿瘤直径、Enneking分期、组织分化差而增高,具有统计学意义(P<0.05)。复发转移组和未复发转移组术前、术后血清VEGF、bFGF表达水平比较,差异存在显著性(P<0.01);经Cox回归多因素分析表明术前和术后血清VEGF、bFGF表达水平是影响患者术后复发转移的独立因素之一(P<0.05)。结论:术前和术后检测骨肉瘤患者血清VEGF、bFGF的表达水平可能对客观预测肿瘤术后转移复发具有一定的参考价值。

AIM： To investigate the correlation between the level of serum VEGF and bFGF with metastasis and recurrence of osteosarcoma. METHODS： 5？, patients with osteosarcoma and 60 healthy people as control group were selected. The serum contents of VEGF and bFGF were determined with ELISA before and after operation. RESULTS： The serum contents of VEGF and bFGF before operation were significantly higher than that in control group （P 〈 0. 01）. The serum contents of VEGF and bFGF after operation descreased significantly but were significantly higher than that in control group（ P 〈 0. 01 ）. The serum contents of VEGF and bFGF before operation were positively related to the size of primarily tumor, Enneking type and tumor differentiation（P〈0. 05）. The serum contents of VEGF and bFGF before and after operation were different significantly between recurrence and metastasis positive group with recurrence and metastasis negative group （ P 〈 0. 01 ）. The Cox proportional hazards model showed that the serum contents of VEGF and bFGF before and after operation were an independent factor of the recurrence and metastasis for osteosarcoma patients after operative （ P 〈 0.01 ）. CONCLUSION： The serum contents of VEGF and bFGF before and after operation can offer valuable assessments in evaluation of progression and metastasis prognosis.