摘要
[Objective] This experiment aimed to evaluate the effects of calcium and Jasmonic acid（JA） on the expression of CBF in spinach. [Methods] The seedlings of spinach were treated with low temperature （4 ℃）, JA or A23187, then used for detecting the expression of CBF by northern blotting. [Results] The results showed that the CBF expression was regulated by low temperature and JA positively. [Conclusions] Low temperature may increase the JA content of the cell firstly, then JA induced the increase of cytosolic calcium concentration （[Ca2＋]cyt）, and the JA induced Ca2＋ transmitted the low temperature signal through CaM or CaM related proteins, regulating the CBF expression.
[目的]为探究钙离子、茉莉酸(Jasmonic acid,JA)对菠菜CBF转录因子表达的影响。[方法]以菠菜(Spinacia oleraceaL.)为材料,对其进行低温(4℃)或JA处理或用钙离子载体A23187预处理菠菜幼苗,利用Northern印迹技术检测各处理时CBF的表达情况。[结果]低温和JA均可诱导菠菜中CBF基因的表达。[结论]推测低温可能首先导致细胞中JA含量升高,JA通过一定的机制诱导细胞质基质中游离钙离子浓度([Ca2+]cyt)升高,然后Ca2+可能通过CaM(Calmodulin)或CaM相关蛋白传递低温信号,从而诱导CBF的表达。
基金
Supported by Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality(PXM2006-014207-021798)
Project of Organization Department of Beijing Municipal Party(20042D0502108)~~
