不同游泳运动方式对大鼠海马cAMP影响的实验研究

The Experimental Study on the Effect of Different Swimming Ways on the Rat Hippocampus's cAMP

目的:观察游泳运动后大鼠海马cAMP浓度变化,探讨不同游泳运动方式对海马cAMP的影响机制。方法:建立大鼠游泳运动模型(持续运动、间歇运动),采用酶联接免疫吸附剂法,观察大鼠海马cAMP浓度变化,并用统计学软件进行数据分析。结果:运动后持续组和间歇组cAMP浓度均呈锯齿形特征,但变化规律有明显差异。持续组先降后升,与对照组相比在即刻、60min、120min、240min有所下降,有显著差异(p<0.05);在60min达到最低值(p<0.01);运动后30min略高于对照组,无显著差异性(p>0.05)。间歇组先升后降且变化幅度逐渐增大,与对照组相比运动后240mincAMP浓度达到峰值,有显著性差异(p<0.01);而运动后30min、120min cAMP浓度呈下降趋势,有显著性差异(p<0.01);运动后即刻、60min cAMP浓度略有上升,无显著差异(p>0.05)。结论:不同游泳运动方式对海马cAMP的影响不同,具有一定的时效性。

Objective： To observe the change of cAMP concentration in rat hippocampus after swimming and to explore different swimming ways＇ effect on the hippocampal cAMP.Methods： To establish a rat swimming model（continued swimming,intermittent swimming）,using enzyme link immunosorbent method to observe the changes of hippocampal cAMP concentration,and use statistical software for data analysis.Results： After exercise the continued group and the intermittent group＇s cAMP concentration all appeared the shape of sawteeth,but there are significant differences in variation.Continued group falls first and then rises,compared with the control group,it declines in the immediate,60 min,120 min,240 min,there are significant differences（p 〈0.05）;in 60 min it reaches the minimum（p 〈0.01）;slightly higher than the control group 30 min after exercise,there was no significant difference（p 〈0.05）.The intermittent group increases first and then decreases gradually and the magnitude changes,as compared with the control group,the cAMP concentration reaches its peak 240 min after exercise,there is a significant difference（P 0.01）;and 30 min,120 min after exercise,cAMP concentration is in a downward trend,there was a significant difference（P 0.01）;The cAMP concentration increases slightly immediately after exercise,60 min,there is no significant difference（p 〈0.05）.Conclusion： Different ways of swimming have different impact on hippocampal cAMP and it has a certain timeliness.