摘要
为了筛选出对人参黑斑病菌具有稳定拮抗作用的微生物,开发新型微生物源农药,本试验用稀释分离法和病组织分离法,从土壤和人参黑斑病菌组织中分离和纯化具有一定拮抗作用的微生物菌株。结果共筛选对人参黑斑病菌有拮抗作用的菌株22个,其中细菌16株,放线菌5株,木霉1株。由此可见,土壤及人参黑斑病组织中均含有对人参黑斑病菌有拮抗作用的微生物菌株,细菌中Xc1、Xd5和Xc4菌株,放线菌中Fd2和Fw1菌株及木霉Zh1都具有开发为防治人参黑斑病微生物源农药的价值。
In order to develop new microbial pesticides with stable antagonistic effect on Ginseng Black Spot,microbial strains with certain antagonistic effects were isolated and purified from soil and Ginseng organization of black spot disease by Dilution Method and Tissue isolation Method in the experiment.The antagonistic effect was assayed by confrontation culture method,and the activities of fermentation liquor from some strains were also determined after fermentation.The results showed that 22 strains have antagonistic effects on title pathogen,including 16 bacteria,5 actinomycetes and 1 trichoderma.From the experiment,microbial strains catching antagonistic effect existed widely in soil and Ginseng organization of black spot disease,such as Xc1,Xd5 and Xc4 in bacteria,Fd2 and Fw1 in actinomycetes and Zh1 in trichoderma.These strains all had potential value on developing microbial pesticides used for the control of Ginseng Black Spot.
出处
《吉林农业科技学院学报》
2011年第3期4-7,共4页
Journal of Jilin Agricultural Science and Technology University
基金
吉林农业科技学院大学生科技创新项目
关键词
人参黑斑病菌
拮抗菌
对峙
发酵液
抑菌效果
ginseng black spot
trichoderma strains
confront
fermentation liquor
antagonistic effect
