丙戊酸钠对人肝癌细胞株Bel-7402增殖及VEGF、bFGF表达的影响

Effect of sodium valproate on proliferation and expression of VEGF and bFGF mRNA in human liver cancer cell line Bel-7402

目的探讨丙戊酸钠(sodium valproate,VPA)对人肝癌Bel-7402细胞的增殖抑制作用及其机制。方法采用MTT比色法检测VPA对Bel-7402细胞增殖的抑制作用;应用流式细胞术(FCM)检测药物作用细胞后细胞凋亡率的变化;通过RT-PCR技术和Western Blot方法检测药物作用细胞后血管内皮生长因子(vascular endothelialgrowth factor,VEGF)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)在mRNA和蛋白水平表达量的改变。结果 VPA对Bel-7402细胞的生长抑制作用呈现时间和剂量依赖性;经0、1、2和4mmol/L VPA作用细胞72小时后,细胞的凋亡率由处理前的(2.78±0.32)%,上升为(8.79±0.53)%、(18.65±1.02)%和(36.41±1.93)%,RT-PCR和Western Blot技术发现VPA作用细胞72小时后,可明显降低VEGF和bFGF的表达,并呈浓度依赖性(P<0.05)。结论 VPA通过下调Bel-7402细胞VEGF和bFGF的表达,对Bel-7402细胞的增殖起抑制作用。

Objective To investigate the inhibiting effect of sodium valproate in human liver cancer Bel-7402 cells and its mechanism.Methods The cell proliferation was determined by MTT assay.Cell apoptosis was analyzed by flow cytometry（FCM） .In addition,the expression level of VEGF and bFGF were analyzed by RT-PCR and Western blot.Results Sodium valproate inhibited the proliferation of Bel-7402 cells in a dose-and time-dependent manner.The apoptotic rate was significantly higher in the cells treated with 1,2 and 4 mmol/L VPA for 72 h than untreated cells [（8.79±0.53） %,（18.65±1.02） %,（36.41±1.93） % vs（2.78±0.32） %].The expression levels of VEGF and bFGF were down-regulated in a dose dependent manner in Bel-7402 treated with VPA for 72 h（P〈0.05） .Conclusion Sodium valproate can inhibit the proliferation of Bel-7402 cells and the possible mechanism is related to the decrease of VEGF and bFGF expression.