中药髓复康对恒河猴脊髓损伤后神经再生的影响

Impacts of Herbal Medicine Suifukang on Nerve Regeneration for Rhesus Monkey with Spinal Cord Injury

目的髓复康对恒河猴脊髓损伤后神经再生的影响。方法成年雄性恒河猴14只,其中10只用于制备T12胸髓左侧半横断损伤模型,并将其随机分为髓复康组(S)和模型组(M),每组5只,另外4只设为正常对照组(N)。通过改良Tarlov法评估恒河猴脊髓神经功能恢复情况;采用原位杂交法检测各组髓鞘相关生长抑制因子(Nogo-A)和生长相关蛋白(GAP-43)的表达量。结果给药30 d后,髓复康组患肢神经功能恢复优于模型组(P<0.01);脊髓损伤后各手术组Nogo-A mR-NA的阳性细胞数及阳性产物的OD值均明显升高,其中髓复康组Nogo-A mRNA的阳性细胞数和其阳性产物的OD值都明显低于模型组(P<0.05)。各手术组GAP-43 mRNA的阳性细胞数及其阳性产物的OD值均明显高于正常组(P<0.01);其中髓复康组GAP-43 mRNA的阳性细胞数和其阳性产物的OD值最高,与模型组比较,差异有统计学意义(P<0.05或P<0.01)。结论中药髓复康能够对脊髓损伤后恒河猴脊髓中Nogo-A的表达有一定的抑制作用,同时能够上调GAP-43的表达,促进了脊髓损伤后恒河猴运动神经功能的恢复。

Objective To explore the impacts of suifukang on nerve regeneration for rhesus monkey with spinal cord injury（SCI）.Methods 14 male adult rhesus monkeys were selected in the experiment.10 monkeys of them were used to prepare the model of left semi-transection of the spinal cord at T12 and they were randomized into a suifukang group and a model group,5 monkeys in each one.Additionally,the rest 4 monkeys were used in a normal control group.The modified Tarlov method was adopted to evaluate the recovery of spinal cord for rhesus monkey.The in situ hybridization（ISH）was applied to detect the expressions of myelin-associated inhibitor factor（Nogo-A）and growth-associated protein（GAP-43）.Results In 30 days of medication,in suifukang group,the nerve function recovery of the affected limb was superior to model group（P0.01）.After SCI,in every operation group,the positive cell number of Nogo-A mRNA and its OD value of positive production all increased apparently,in which,the positive cell number of Nogo-A mRNA and its OD value of positive production in suifukang group was lower obviously than those in model group（P0.05）.The positive cell number of GAP-43 mRNA and its OD value of positive production were higher obviously than those in normal group（P0.01）,in which,the positive cell number of GAP-43 mRNA and its OD value of positive production were the highest in suifukang group,presenting significant difference as compared with model group（P0.05 or P0.01）.Conclusion The herbal medicine suifukang inhibits Nogo-A expression to a certain extent and up-regulates GAP-43 expression in spinal cord,and promotes the recovery of motor nerve for rhesus monkey with spinal cord injury.