加味聚精食疗方对少、弱精子症大鼠EGF、EGFR睾丸内表达的影响

Impacts of Modified Jujing Dietetic Recipe on the Expressions of EGF and EGFR in the testis of the Rats with Oligospermatism and Athenospermia

目的观察加味聚精食疗方对少、弱精子症大鼠表皮生长因子(EGF)、表皮生长因子受体(EGFR)睾丸内表达的影响。方法 60只3月龄雄性SD大鼠采取随机化法分为六组:G1组、G2组、G3组、G4组、G5组、G6组,每组10只。G1组蒸馏水灌胃52 d;其余五组予雷公藤多苷(GTW)灌胃26 d造模。造模结束后,G6组立即脱颈处死,以了解造模质量;G2组加味聚精食疗方灌胃;G3组枸橼酸他莫昔芬片灌胃;G4组五子衍宗片灌胃;G5组蒸馏水灌胃,以上均每天1次,连续26 d。治疗结束后CASA技术检测大鼠附睾精子质量;Envision两步法检测睾丸组织EGF、EGFR的表达。结果G6组附睾精子质量的各项指标均较G1组明显减低(P<0.05),显示造模成功。G2组附睾精子质量所有指标均明显优于G5、G6组(P<0.05)。G2组附睾精子密度明显优于G3、G4组(P<0.05)。G1、G2组附睾精子质量所有指标组间比较,差异无统计学意义(P>0.05)。G1、G2组大鼠睾丸组织EGF、EGFR表达水平明显优于G5、G6组(P<0.05)。结论加味聚精食疗方能显著上调大鼠睾丸间质细胞EGF、EGFR的表达水平,改善精子质量。

Objective To observe the impacts of modified juying dietetic recipe on the expressions of epidermal growth factor(EGF)and epidermal growth factor receptor(EGFR)in the testis of the rat with oligospermatism and athenospermia.Methods 60 SD male rats of 3 months old were randomized into six groups,named G1 group,G2 group,G3 group,G4 group,G5 group and G6 group,10 rats in each one.In G1 group,the gastric infusion with distilled water was done for 52 days.In the rest five groups,tripterygium glucosides(GTW)was used in gastric infusion for 26 days for modeling.At the end of modeling,the rats in G6 were sacrificed for the determination of modeling quality.In G2 group,the modified juying dietetic recipe was used in gastric infusion;In G3,tamoxifen citrate tablets were for gastric infusion;in G4 group,wuzi yanzu tablets were for gastric infusion;in G5,distilled water was for gastric infusion.All the above gastric infusions were done once per day,continuously for 26 days.At the end of treatment,CASA technology was adopted to detect the sperm quality in the epididymis of rat;Envision two-step method was used to detect the expressions of EGF and EGFR in testis tissue.Results Every index of sperm quality in the epididymis in G6 was reduced apparently as compared with G1 group(P<0.05),indicating successful modeling.All the indexes of sperm quality in the epididymis in G2 group were superior to G5 and G6 groups(P<0.05).The sperm density in the epididymis in G2 group was apparently superior to G3 and G4 group(P<0.05).There was no statistical significance of the difference in all the indexes of sperm quality in the epididymis between G1 and G2 groups.The expressions of EGF and EGFR in testis tissue in G1 and G2 were apparently superior to G5 and G6 groups.Conclusion The modified juying dietetic recipe up-regulates EGF and EGFR expressions in Leydig cells and improves sperm quality significantly in rats.