微波辅助法提取野生软枣猕猴桃茎黄酮的工艺优化

Optimization of microwave-assisted extraction of flavonoids from Actinidia arguta stem

研究了微波辅助法提取野生软枣猕猴桃茎黄酮类化合物的工艺条件。通过单因素实验分别考察微波功率、提取时间、乙醇浓度、料液比对黄酮提取率的影响,以软枣猕猴桃茎黄酮的提取率为指标,根据单因素实验结果设计正交实验,得出微波辅助法提取的最佳工艺条件:微波功率为300W,乙醇浓度为70%,料液比为1∶32,提取时间为5min,此条件下软枣猕猴桃茎黄酮提取率为1.80%。

Microwave-assisted extraction of flavonoids from the stem of Actinidia arguta was explored. Effect of operation conditions on extraction ratio of flavonoids,such as microwave power,treatment time,ethanol concentration and liquid ratio were studied by using single factor test. Taking extraction ratio of flavonoids as index,orthogonal test was designed based on single factor test. Results showed that the highest extraction yield can be obtained when microwave power was 300W,treatment time was 5min,ethanol concentration was 70% and liquid ratio was 1∶ 32. Under the optimal extraction conditions,the extraction rate of flavonoids from Actinidia arguta stem was 1. 80%.