高效液相色谱法测定表没食子儿茶素没食子酸酯含量

Determination of epigallocatechin gallate in green tea extract by high performance liquid chromatography

建立了一种利用高效液相色谱法(HPLC)测定绿茶提取物原料中表没食子儿茶素没食子酸酯(EGCG)含量的方法。通过对流动相组成、色谱柱、流速及温度等色谱条件的选择及优化,得到了适合EGCG检测的色谱条件:色谱柱TSK-GELODS-100V,流动相组成甲醇∶水∶乙酸=23∶75∶2的,流速1.0mL/min,检测波长276nm,柱温24℃。实验结果表明,EGCG在含有咖啡因及与EGCG类似物质的复杂体系中被快速、高效地检出。同时,该方法操作简单,检测效率高,重现性好,可以有效地节约实验成本,进一步证明了该方法可行性。在实际测定中,可以实现原料检测工作的快速、高效运行,适合企业对绿茶提取物原料中EGCG的质量控制。

A method for determination of EGCG by HPLC in green tea extract was developed. The chromatographic conditions including mobile phase composition,chromatographic column,flow rate and temperature were optimized. Exactly,TSK-GELODS-100V column was used with the mobile phase of methanol：water：aceticacid（23∶ 75∶ 2）at column temperature of 24℃,and the flow rate was 1. 0mL/min with UV detection at 276nm. The results showed that EGCG was rapidlly separated and detected in the complex system contained caffeine and EGCG similarities. With the advantages of simple,low cost,high efficency and good reproducibility,this method could be successfully used for quality control in the determination of EGCG in green tea extract.