CatWalk步态检测方法在大鼠脑损伤模型运动功能评估中的应用

CatWalk-assisted gait test in evaluating motor funtion of rat models of traumatic brain injury

目的探讨CatWalk步态检测方法在评价大鼠脑损伤四肢运动功能改变中的应用价值。方法采用改良的Feeney自由落体脑损伤装置制造中度大鼠颅脑损伤模型，损伤部位均为大鼠右侧大脑皮层。损伤前及损伤后的第3、7、14、28天，对模型鼠进行CatWalk步态变化检测及改良神经功能缺损评分（mNSS），并同步记录相应结果。结果损伤后第7天，大鼠左后肢的各参数与损伤前相比．差异有统计学意义（P〈0．05）；大鼠左右后肢间的比较差异亦有统计学意义（P〈0．05）；损伤后第14天，大鼠行走循环、平均强度、两步之间爪子离开平板的时间与损伤前比较差异有统计学意义（P〈0．05）；大鼠左右前肢间的运动参数（最大接触面积、接触强度、爪印长度、行走循环）与损伤前比较差异有统计学意义f氏0．05）。第3天及28天大鼠各项运动参数差异均无统计学意义（P〉0．05）。损伤前、损伤后第3、7、14、28天大鼠mNSS评分差异有统计学意义（P〈0．05），损伤后第3，7天评分明显高于损伤前。结论CatWalk检测能准确反映出大鼠运动功能的变化，对于研究脑损伤模型大鼠行为学改变有重要意义。

Objective To explore the value of CatWalk-assisted gait test in evaluating the changes of motor function in the rat models of traumatic brain injury. Methods Improved Feeney free fall brain damage device was employed to establish the craniocerebral injury rat models; injured loci were located in the right side of the brain cortex; CatWalk-assisted gait test and mNSS were performed on the 3rd, 7th, 14th and 28th d of injury. SPSS 13.0 software was used to compare the scores of the above tests. Results Significant differences on each parameter of the left hind limb were noted between the day before injury and the day of 7 d after injury （P〈0.05）; and significant differences were also noted between left and right hind limbs （P〈0.05）. Significant differences on such motion parameters as maximum contact area, contact strength, claw length and walking cycle in the left and right fore limbs were noted between the 14th d of injury and before injury（P〈0.05）; and the walking cycle, average intensity and time of claws leaving the flat between two steps in these rats were signficantly different between the 14th d of injury and before injury （P〈0.05. No significant differences on each motion parameter were found between the 3rd and 28th d of injury （P〉0.05）. Significant differences on mNSS scores were noted between the day before injury and the 3rd, 7th 14th, and 28a d of injury （P〈0.05）; and the mNSS scores on the 3rd and 7th d of injury were obviously higher than those before injury. Conclusion Catwalk-assisted gait test can accurately detect the changes of motor function, enjoying an important significance in the study of behavior of rat with traumatic brain injury.