小檗碱对阿尔茨海默病转基因鼠空间记忆力的影响

Effect of berberine on spatial memory in transgenic mice with Alzheimer＇s disease

目的探讨小檗碱对AD转基因鼠空间记忆力的影响。方法24只6月龄AD转基因鼠按随机数字表法分为模型组、小檗碱小剂量组、小檗碱中剂量组和小檗碱大剂量组，后三组小鼠分别每天灌胃给予小檗碱10mg／（kg·d）、50mg／（kg·d）和200mg／（kg·d）N10月龄。通过Morris水迷宫试验和ELISA法检测不同剂量小檗碱对AD转基因鼠空间记忆力和脑组织B淀粉样蛋白40、42（Aβ40、Aβ42）水平的影响。结果与模型组相比，各小檗碱组小鼠逃避潜伏期在测试第6天时均明显缩短．穿越平台的次数均明显增加，脑组织Aβ40、Aβ42水平均明显降低，差异均有统计学意义伊〈0．051结论小檗碱可能抑制小鼠脑组织Aβ40、Aβ42的产生从而改善AD转基因鼠的空间记忆力。

Objective To investigate the effect ofberberine on the spatial memory oftransgenic mice with Alzheimer＇s disease （caused by Swedish double mutation （KM670/671NL） of amyloid precursor protein [Swe-APP]）. Methods Twenty-four 6-month-old APP/SWE mice were randomly divided into 4 groups： model group, low-dose berberine treatment group, medium-dose berberine treatment group and high-dose berberine treatment group （n=6）. Except mice of the model group, the mice in the other 3 groups were given berberine at dosage of 10 （mg/kg .d）, 50 （mg/kg.d） and 200 （mg/kg .d）, respectively, by intragastric administration once daily to 10 months＇ old. The spatial memory was detected by Morris water maze test, and the levels of amyloid β （Aβ40 and Aβ42） in the brain tissues were measured by enzyme linked immunosorbent assay （ELISA）. Results As compared those in mice of the model group, the escape latency decreased significantly, the frequency of crossing the platform obviously increased and the levels of Aβ40 and Aβ42 decreased significantly in mice of each berberine treatment group on the 64 d of detection （P〈0.05） Conclusion Berberine might improve the spatial memory of APP/SWE mice by decreasing the levels of Aβ 40/42.