脾酪氨酸激酶在不同等级胶质瘤中的表达及其与周期素1水平的相关性

Expression of spleen tyrosine kinase in glioma of different grades and its relation with CyclinD1 level

目的探讨人脑胶质瘤中脾酪氨酸激酶（Syk）的表达及其与周期素1（CyclinD1）水平的相关性。方法收集贵州航天医院神经外科自2005年1月至2010年1月间手术切除并经病理证实的脑胶质瘤标本46例，其中Ⅰ级13例，Ⅱ级9例，Ⅲ级5例，Ⅳ级胶质母细胞瘤19例。另取5例因脑创伤行内减压术患者的正常脑组织标本作为对照，RT-PCR检测脑组织标本SykmRNA、CyclinD1 mRNA的表达并分析二者的相关性。结果与正常脑组织比较，Ⅰ、Ⅱ、Ⅲ、Ⅳ级脑胶质细胞瘤标本SykmRNA表达较低，CyclinD1mRNA表达较高，差异有统计学意义（P〈0．05），而且胶质瘤的病理级别越高，SykmRNA的表达越低，CyclinD1 mRNA表达较高，差异均有统计学意义（P〈0．051；胶质瘤中Syk与CyclinD1的表达呈负相关关系（r=-0．832，P=-0．000）。结论Syk在脑胶质瘤中表达较低或缺失，提示其可能具有抑癌基因功能，其机制可能与下调胶质瘤中CyclinD1的表达有关。

Objective To explore the human brain spleen tyrosine kinase （Syk） expression and its correlation with CyclinD1 level. Methods Forty-six glioma samples, confirmed by pathology and performed surgical resection in our hospital from January 2005 to January 2010, including 13 of grade Ⅰ, 9 of grade Ⅱ, 5 of grade Ⅲ and 19 with glioblastoma multiforme of grade Ⅳ, were chosen; another 5 normal brain tissues from patients with cerebral trauma were chosen as controls. RT-PCR was employed to detect the mRNA levels of Syk and CyclinD1; and their correlation was analyzed. Results As compared with the normal brain tissues, glioma tissues of grade Ⅰ, Ⅱ, Ⅲ and Ⅳ enjoyed significantly lower mRNA level of Syk and obviously higher mRNA level of CyclinD1 （P〈0.05）. The mRNA level of Syk was significantly decreased with the increase of pathological grades, while the mRNA level of CyclinD1 was in the opposite position （P〈0.05）; Expression level of Syk in gliomas was negatively significantly correlated with that of CyclinD1 （r=-0.832, P=0.000）. Conclusion Decreased or missed Syk expression in glioma might suggest that it may have a tumor suppressor gene function, which might be related to decreased expression of CyclinD1 in glioma.