禾本红酵母Y-5对结晶紫、孔雀石绿的吸附

Biosorption of crystal violet and malachite green by Rhodotorula graminis Y-5.

研究了培养时间、初始pH、温度对禾本红酵母Y-5吸附结晶紫、孔雀石绿的影响,并对吸附剂的解吸和循环利用等进行考察.结果表明:在染料浓度为50mg.L-1、pH7.0、摇床转速150r.min-1、30℃、吸附10h时,红酵母对结晶紫、孔雀石绿的吸附率分别达峰值,为93.8%和87.7%;解吸后的菌体对结晶紫、孔雀石绿的吸附率分别为85.5%和78.5%,说明菌体对染料的吸附是可逆的,循环利用效果良好,菌体可再生和循环利用;禾本红酵母Y-5对结晶紫、孔雀石绿的脱色机理为吸附作用,染料多被吸附在红酵母表面的羟基(-OH)上,其吸附染料快速、高效、可逆,在染料废水处理上具有潜在的应用价值.

With a shaker,this paper studied the characteristics of the biosorption of crystal violet and malachite green by Rhodotorula graminis Y-5 under different adsorption time,initial pH,and temperature,as well as the desorption and recycling use of the dyes.The biosorption of crystal violet and malachite green by R.graminis Y-5 had the peaks（93.8% and 87.7%,respectively） at pH 7.0,dye concentration 50 mg·L-1,150 r·min-1,30 ℃,and lasting 10 hours.After desorption,the biosorption rate of crystal violet and malachite green by R.graminis was 85.5% and 78.5%,respectively,indicating that the biosorption of crystal violet and malachite green was reversible,and the recycling use of the dyes by R.graminis was quite good,i.e.,the dyes were renewable and could be recycled.Biosorption could be the mechanism of the decolorization of the dyes.The dyes were mostly adsorbed on the R.graminis surface-OH.The adsorption process was fast,efficient,and reversible,suggesting that R.graminis had a high potential for waste water treatment.