广东省2009年副溶血弧菌暴发与散发菌株的病原学特征分析

Etiologic characteristics of Vibrio parahaemolyticus strains causing outbreaks and sporadic cases in Guangdong, 2009

目的了解2009年广东省副溶血弧菌食物中毒分离株和腹泻患者监测分离株的血清分型、毒力基因携带情况及分子特征。方法对95株副溶血弧菌食物中毒分离株和15株腹泻患者分离株进行血清分型，耐热直接溶血素相关基因（trh）和耐热直接溶血素基因（tdh）PCR检测以及选取不同血清型副溶血弧菌81株进行脉冲场凝胶电泳（PFGE）分子分型。结果从监测腹泻患者分离的15株副溶血弧菌，血清型以03：K6（46．67％）和04：K8（33．33％）为主；从11起副溶血弧菌食物中毒分离的95株菌，血清型以03：K6（44．21％）和04：K8（28．42％）居多；7株食品分离株都不是03：K6血清型；93株（84．54％）为tdh＋trh菌株，13株（11．81％）为tdh-trh-菌株，3株（3．65％）为tdh＋trh＋菌株。81株副溶血弧菌的PFGE相似值为57．7％～100．0％，被分为36种不同的PFGE型别，PFGE001型和029型为2009年广东省副溶血弧菌优势PFGE型别。结论2009年广东省引起感染性腹泻和食物中毒的副溶血弧菌以03：K6和04：K8型为主要血清型，多数菌株携带tdh基因，存在优势PFGE型别菌株不断引起各地区的散发和暴发。

Objective To study the serotypes, virulence features and molecular characteristics of Vibrio parahaemolyticus isolated in food poisoning cases and surveillance program on diarrhea patients in Guangdong, 2009. Methods 95 Vibrio parahaemolyticus strains from food poisoning cases and 15 strains from surveillance program on diarrhea patients were serotyped and detected for tdh （thermostable direct hemolysin, tdh） and trh （tdh- related hemolysin gene, trh） by PCR. 81 sero-variant Vibrio parahaemolyticus strains were selected through PFGE subtyping. Results There were 15 Vibrio parahaemolyticus strains isolated from surveillance program on diarrhea patients and 95 strains were isolated from ll Vibrio parahaemolyticus- caused food poisoning cases in 2009. Among these strains, 03 ： K6 （46.67% and 44.21% ） and 04 ： K8 （33.33% and 28.42% ） were the dominant serotypes, but not the 7 food-borne strains. There were 93 （ 84.54% ） tdh ＋ trh-, 13 （ 11.81% ） tdh-trh , and 3 （3.65%） tdh＋trh＋ strains. The similarity value was between 57.7% to 100.0% of the 81 strains after PFGE sub-typing method and 36 PFGE subtypes were identified. PFGE001 and PFGE029 appeared to be the dominant subtypes. Conclusion 03 ： K6 and 04 ： K8 were the most dominant serotypes in Vibrio parahaemolyticus-caused diarrhea and food poisoning cases in Guangdong and tdh were detected in most of the strains. Dominant PFGE subtypes were causing both sporadic and outbreak cases in different areas in Guangdong province.