中性粒细胞碱性磷酸酶染色对淋巴瘤病理分型及病情评估的意义

Significance of neutrophilic alkaline phosphatase stain on pathology typing and prognosis evaluation of lymphoma

目的:探讨中性粒细胞碱性磷酸酶(NAP)染色对淋巴瘤病理分型及病情评估的意义。方法:采用偶氮偶联法对66例初诊的淋巴瘤患者进行外周血中NAP的监测,并与40例正常人(正常对照组)进行比较。结果:(1)与正常对照组相比,B细胞淋巴瘤NAP增高,T细胞淋巴瘤NAP减低,但差异无统计学意义(P>0.05);与T细胞淋巴瘤相比,B细胞淋巴瘤NAP明显增高(P<0.05)。(2)各病理类型B细胞淋巴瘤间NAP差异无显著性,伴巨大包块的B细胞淋巴瘤NAP明显减低(P<0.01)。(3)各组弥漫性大B细胞淋巴瘤间NAP差异无显著性。(4)骨髓侵犯组B细胞淋巴瘤较非骨髓侵犯组B细胞淋巴瘤NAP明显减低(P<0.01),骨髓侵犯组T细胞淋巴瘤较非骨髓侵犯组T细胞淋巴瘤NAP明显增高(P<0.01)。(5)随疾病进展,B细胞淋巴瘤NAP呈下降趋势,T细胞淋巴瘤NAP呈上升趋势;随疾病好转,B细胞淋巴瘤NAP呈上升趋势,T细胞淋巴瘤NAP呈下降趋势。结论:NAP染色对淋巴瘤病理分型及预后的判断均具有一定的临床价值。

Objective To explore the significance of neutrophilic alkaline phosphatase （NAP） stain on pathology typing and prognosis evaluation of lymphoma. Methods Peripheral blood NAP stain were monitored in 66 cases of preliminal）- diagnosis of lymphoma. Results （ 1 ）Contrast to normal control, NAP in B cell lymphoma was higher, while NAP in T cell lymphoma was lower. NAP in B cell lymphoma was obviously higher than in T cell lymphoma （P 〈 0.05）. （2）NAP among each pathology typing of B cell lymphoma was no difference. NAP in B cell lymphoma with large mass was obviously lower than in B cell lymphoma without large mass （P 〈 0.01 ）. （3）NAP in each group of diffuse large B cell lymphoma was no difference. （4）NAP in B cell lymphoma with bone marrow invasion was obviously lower than in B cell lymphoma without bone marrow invasion, which was opposite in T cell lymphoma （P 〈 0.01 ）. （5）NAP was descending following disease progression in B cell [ymphoma, which was opposite in T cell lymphoma. While NAP was rising following disease improvement in B cell lymphoma, which was opposite in T cell lymphoma. Conclusion NAP stain was significant to pathology typing and prognosis evaluation of lymphoma.