吗啡对HIV-1在MT2细胞内复制影响

Effect of morphine on replication of HIV-1 in MT-2 cells

目的探讨吗啡对人类免疫缺陷病毒-1(HIV-1)在MT2细胞中复制的影响。方法将MT2细胞按单纯随机分组的原则分为吗啡处理组、吗啡+纳洛酮处理组、纳洛酮处理组和病毒对照组,先用浓度为10-8mol/L的纳洛酮预处理吗啡+纳洛酮处理组和纳洛酮处理组0.5 h,再用10-12、10-10和10-8mol/L 3个浓度的吗啡处理吗啡+纳洛酮处理组和吗啡处理组24 h,然后每组细胞加入HIV-1感染MT2细胞,并分别于感染的第3、4、5和6 d取培养上清测定HIV-1 p24抗原表达。结果 HIV-1感染MT2细胞第3、4、5、6 d,10-12、10-10和10-8mol/L 3个浓度吗啡处理组的HIV-1 p24抗原表达均高于病毒对照组,差异均有统计学意义(P<0.01);吗啡+纳洛酮处理组、纳洛酮处理组与病毒对照组HIV-1 p24抗原表达比较,差异均无统计学意义(P>0.05);第3、4、5、6 d,3个浓度吗啡处理组的HIV-1 p24抗原表达量比对照组增加的倍数比较,差异均有统计学意义(P<0.05);10-12、10-10和10-8mol/L 3个浓度吗啡处理组在不同时间HIV-1 p24抗原表达量比对照组增加倍数比较,3个吗啡处理组的HIV-1 p24抗原表达量比对照组增加倍数均随着感染时间的延长而呈现递增的趋势(P<0.05)。结论吗啡能够促进HIV-1在MT2细胞和内的复制,并且随感染时间的延长呈现递增趋势;吗啡促进HIV-1复制的作用可被阿片受体阻滞剂纳洛酮阻断。

Objective To determine whether morphine has the ability to enhance HIV-1 replication in MT-2 cells in vitro.Methods MT-2 cells were random assigned into morphine treatment group,morphine/naloxone co-treatment group,naloxone treatment group,and control group.The MT-2 cells in treatment groups were treated with morphine and/or naloxone for 24 hours,and then infected with HIV-1 strain.HIV-1 p24 antigen in MT2 cells culture supernatant collected at days 3,4,5 and 6 after the infection was determined.Results For the MT-2 cells infected with HIV-1 on the 3rd,4th,5th,and 6th day,the expressions of HIV-1 p24 antigen in 10-12mol/L,10-10mol/L and 10-8mol/L concentration of morphine treatment group were increased significantly compared to those of the control（P0.01 for all）.There was no significant difference in the expression of HIV-1 p24 antigen among morphine/naloxone co-treatment group,naloxone treatment group and control group（P0.05 for all）.When MT-2 cells infected with HIV-1 on the 3rd,4th,5th,and 6th,the p24 antigen expressions were enhanced remarkably with significant difference among different concentration morphine treatment groups（P0.05）.There were significant differences for different infection time in the p24 antigen expressions of three concentration of morphine treatment groups（P0.05）,with an increase trend with the duration HIV-1 infection.Conclusion Morphine has the ability to enhance HIV-1 replication in MT-2 cell and the enhancement increases with the duration of HIV-1 infection.The morphine-mediated increase of HIV-1 p24 antigen expression could be blocked by the opiate receptor antagonist（naloxone）.