摘要
本文建立了饲料中赭曲霉毒素A(OTA)的免疫亲和柱净化-高效液相色谱荧光检测方法。样品用甲醇-水(体积比80∶20)提取,通过免疫亲和柱富集和净化,以乙腈和2%乙酸溶液为流动相(体积比44∶56),Agilent C18色谱柱分离,荧光检测器检测(λex-332 nm,λem=460 nm)。OTA的线性范围为0.1~100.0 ng/mL,r=0.99989。方法检测限为0.2μg/kg,定量限为0.6μg/kg。样品中赭曲霉毒素A的加标回收率为75%~92%,相对标准偏差为1.65%~3.61%。
A method was established for the determination of ochratoxin A(OTA) in feed by high performance liquid chromatography (HPLC) technique, with immunoaffinity column (IAC)pre-separation and fluorescence detection (FLD). Feed samples were first extracted with methanol-water (80:20, V/V) solution.Then,the extract was pre-separated and concentrated by the using of IAC.The optimized mobile phase was mixture of acetonitrile and 2 % acetic acid (44:56, V/ V) ,the analysis was performed on an Agilent C18 column, and quantified with fluorescence detection (λex=332 nm, λem= 460 nm).Linear response for OTA was observed in the range of 0.1 - 100.0 ng/mL (r=0.99989).The limit of detection (LOD) and the quantization (LOQ) were 0.2 ug/kg and 0.6 ug/kg,respectively.Recoveries of 75% - 92% were observed for the feed spiked with OTA,and the relative standard deviation was 1.65 % - 3.61%.
出处
《中国饲料》
北大核心
2011年第23期38-40,共3页
China Feed
关键词
免疫亲和柱
高效液相色谱
饲料
测定
immunoaffinity column
high performance liquid chromatography (HPLC)
feed
determination