硫酸铟对V79细胞内活性氧和线粒体膜电位的影响

Effects of Indium Sulfate on Intracellular Reactive Oxygen Species and Mitochondrial Membrane Potential in V79 Cells

[目的]观察硫酸铟对中国仓鼠肺纤维细胞(V79细胞)内活性氧(ROS)和线粒体膜电位的影响。[方法]不同浓度硫酸铟(0.5、1.0、2.0、4.0、8.0mmol/L)染毒体外培养的V79细胞,MTT法检测细胞存活率;以2’,7’-荧光素乙二酯和碘化四氯代四乙基苯咪唑羰花青为探针,采用流式细胞术,检测经不同浓度硫酸铟染毒12h后V79细胞内ROS和线粒体膜电位的水平。[结果]硫酸铟能明显降低V79细胞的存活率;不同浓度硫酸铟作用于V79细胞12h后,细胞内的平均荧光强度从21.60增加到31.10,线粒体膜电位从0.1913降低到0.1090,与对照组相比,差异有统计学意义(P<0.05)。[结论]硫酸铟能增加V79细胞内ROS的释放、降低线粒体膜电位,影响细胞的正常代谢功能,对细胞造成损伤。

[Objective] To observe the changes of intracellular reactive oxygen species（ROS） and mitochondrial membrane potential（Δψm） in Chinese hamster V79 cells induced by indium sulfate in vitro.[Methods] The cultured V79 cells were exposed to indium sulfate at various dosages（0.5,1.0,2.0,4.0 and 8.0 mmol/L）.The cell survival rate was checked with methyl thiazolyl tetrazolium（MTT）.The changes of intracellular ROS and Δψm in V79 cells were analyzed by flow cytometry with 2’,7’-dichlorodihydrofluorescin diacetate（DCFH-DA） and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide（JC-1） probe after being interfered for 12h.[Results] Indium sulfate significantly reduced the survival rate of V79 cells.After exposed to indium sulfate at various dosages for 12h,the mean intracellular fluorescence intensity obviously increased from 21.60 to 31.10 while the Δψm significantly decreased from 0.1913 to 0.1090.The statistical differences were significant between the control group and the exposure groups（P0.05）.[Conclusion] Indium sulfate can increase the release of intracellular ROS and reduce the Δψm significantly.The damage of indium sulfate to V79 cells might be associated with the change of normal cellular metabolism.