诱导性多能干细胞移植重建造血衰竭小鼠模型造血功能的研究

Reconstitution of hematopoiesis in mice with hematopoietic failure by transplantation of induced multipotent stem cells

目的探讨诱导性多能干细胞对造血衰竭小鼠模型进行造血重建的作用。方法建立雌性小鼠造血衰竭模型,移植经天然活性物质诱导的雄性小鼠脾细胞,检测受体小鼠生存时间、外周血WBC、脾湿重及脾集落(CFU-S)数,进行G带染色体分析受体小鼠是否有Y染色体。结果经天然活性物质诱导的雄性小鼠脾细胞5×106个输注给致死剂量照射后的雌性小鼠,可明显延长其存活时间,提高受体小鼠外周血WBC数,也能增加脾湿重,提高脾集落数,经G带染色体分析进行Y染色体检测,受体小鼠骨髓检测可观察到20%的Y染色体。结论经天然活性物质诱导的小鼠脾细胞含有多能干细胞,回输注入造血衰竭的小鼠能重建小鼠造血功能。

Objective To study the effectiveness of induced multipotent stem cells for the reconstitution of hematopoiesis in mice with hematopoietic failure.Methods Female mice models of hematopoietic failure were established and were transplanted with natural active substances-induced spleen cells of male mice.Survival time,peripheral WBC,spleen wet weight and spleen colony number of recipient mice were detected,and the G band chromosome analysis was processed to determine whether Y chromosome could be detected in female mice models.Results 5×106 natural active substances-induced male mice spleen cells were infused to lethally irradiated female mice,and could significantly prolong survival time,increase the number of WBC in peripheral blood of recipient mice,increase the spleen wet weight and numbers of spleen colony（CFU-S）.Chromosome analysis by G band staining indicated that the positive rate of Y chromosome in recipient mice was 20%.Conclusion Natural active substances-induced spleen cells could contain multipotent stem cells,and could reconstitute hematopoietic function in mice with hematopoietic failure by infusion.