摘要
目的探讨Notch-1基因在人大肠癌中的表达水平,并探讨其在大肠癌发生、发展中的作用。方法采用RT-PCR和Western blot方法检测大肠癌组织和对照组织标本中Notch-1的表达水平。结果 Notch-1在癌组织与对照组织中均有表达,但在癌组织中的表达明显高于对照组织,两者差异有统计学意义。结论 Notch-1基因在大肠癌中的表达水平明显增高,提示在结直肠中,Notch-1高表达对大肠癌的发生和发展具有促进作用。
Objective To evaluate the expression of Notch-l gene and its mechanisms in the development of cancer of colon.Methods The levels of Notch-1 gene in tissues of cancer of colon and normal colon were measured by RT-PCR and Western blot.Results The expression of Notch-l gene could be detected both in tissues of cancer of colon and normal colon.The expression of Notch-l was significantly up-regulated in cancer of colon,compared with normal colon tissues（P0.05）.Conclusion The expression of Notch-l in cancer of colon was significantly higher than that in normal tissues,indicating that the expression of Notch-1 might be correlated with the carcinogenesis and development of cancer of colon.
出处
《国际检验医学杂志》
CAS
2011年第18期2055-2056,共2页
International Journal of Laboratory Medicine
