乙型肝炎X蛋白的检测及其对乙型病毒性肝炎影响的探讨

Detection of HBV X protein and its influence on viral hepatitis B

目的通过检测乙型肝炎患者的X蛋白,探讨其对疾病的影响。方法采用酶联免疫吸附试验(ELISA)检测乙型肝炎患者血清中X蛋白,分别比较健康对照组、肝癌组与肝炎组的X蛋白检测阳性率的差异;分析乙型肝炎DNA测定值阳性和阴性中X蛋白阳性率的差异以及e抗原(HBeAg)阳性和阴性中X蛋白检出率的差异,探讨乙型肝炎X蛋白对疾病的影响。结果肝炎组患者的X蛋白阳性率高于健康对照组,肝癌组X蛋白阳性率高于肝炎组,乙型肝炎DNA测定值阳性X蛋白阳性率高于阴性,HBeAg抗原阳性中X蛋白的检出率也高于阴性,并且差异均有统计学意义。结论乙型肝炎X蛋白在疾病监测中有一定的临床意义,并且可能对病毒的复制有影响。

Objective To detect HBV X protein in patients with viral hepatitis B to explore its influence on disease.Methods The difference of the positive rate of HBV X protein,detected by enzyme linked immunosorbent assay（ELISA） in healthy controls,patients with hepatocelluar carcinoma and patients with viral hepatitis B.The positive rates of HBV X protein in subjects positive or negative with HBV DNA or HBeAg were also compared.Results The positive rate of HBV X protein in patients with viral hepatitis B was significantly higher than in healthy controls,in patients with hepatocelluar carcinoma was significantly higher than in patients with viral hepatitis B,in subjects positive with HBV DNA or HBeAg was significantly higher than in those negative with HBV DNA or HBeAg respectively.Conclusion HBV X protein could have certain clinical significance for the monitoring of disease and might affect the duplication of HBV.