肝脏组织S-腺苷甲硫氨酸酶与卡氏肺孢子虫肺炎的相关性研究

Research on the correlation between S-adenosylmethionine enzyme in liver tissue and Pneumocystis carinii pneumonia

目的探讨卡氏肺孢子虫肺炎大鼠模型肝脏组织S-腺苷甲硫氨酸酶的变化。方法给SD大鼠皮下注射地塞米松建立肺孢子虫肺炎动物模型,采用改良的六亚甲基四胺银染色法检测获得肺组织中的肺孢子虫包囊,利用组织S-腺苷甲硫氨酸酶检测试剂盒测定肝脏组织中S-腺苷甲硫氨酸酶的含量。结果感染组大鼠全部感染了卡氏肺孢子虫,感染率为100%;肝脏组织中S-腺苷甲硫氨酸酶的含量为(0.254 5±0.067 9)μmol/mg,正常对照组大鼠卡氏肺孢子虫包囊的检出率为0%,肝脏组织中S-腺苷甲硫氨酸酶的含量为(0.401 4±0.087 8)μmol/mg,感染组与正常对照组比较,差异有统计学意义(P<0.05)。结论卡氏肺孢子虫肺炎大鼠肝脏组织中S-腺苷甲硫氨酸酶的含量明显降低,提示测定肝脏组织S-腺苷甲硫氨酸酶的含量可以辅助诊断卡氏肺孢子虫肺炎。

Objective To study the changes of S-adenosylmethionine enzyme in liver tissues of rats with Pneumocystis carinii pneumonia.Methods Sprague-Dawley（SD） rats were subcutaneously injected with dexamethasone sodium phosphate to establish animal model of Pneumocystis carinii（P.carinii） pneumonia.The cysts of P.carinii in the lung tissues were observed by staining with the improvement gormori methenamine silver（GMS）,and the content of liver S-adenosylmethionine enzyme was detected by using AdoMet kit.Results All rats of infection group were infected with P.carinii,the content of liver S-adenosylmethionine enzyme of which was 0.254 5±0.067 9 μmol.No rat of control group was infected with P.carinii,and the content of liver S-adenosylmethionine enzyme in control group was 0.401 4±0.087 8 μmol.There was statistical difference of the content of liver S-adenosylmethionine enzyme between the two groups（P0.05）.Conclusion The content of liver S-adenosylmethionine enzyme in rats with P.carinii pneumonia reduced obviously,indicating that detecting the content of liver S-adenosylmethionine enzyme could be used for the auxiliary diagnosis of P.carinii pneumonia.