一株高产黑色素暗盘菌菌株的选育

Breeding of a High Melanin-producing Plectania Strain

研究黑色素暗盘菌Plectania YM421原生质体制备的最佳条件,并对该原生质体进行紫外诱变以获得高产黑色素菌株。结果表明:在质量浓度为2.5g/100mL蜗牛酶,33℃酶解3h的条件下,Plectania YM421原生质体形成率最高;在距离20W紫外灯下30cm处,紫外诱变时间在15～25s之间时,获得突变株YM421-2#黑色素的产量较出发菌株提高了10.03%,生物量提高了11.33%。该突变株可以作为黑色素工业生产的候选菌株。

In order to breed a Plectania strain with higher melanin-producing ability,the preparation and UV mutagenization of protoplasts from Plectania YM421 mycelium was optimized.Results indicated that maximum rate of protoplast formation was observed after 3 h hydrolysis of helicase added at a level of 2.5 g/100 mL at 33 ℃.After exposure to UV radiation for 15-25 s at the distance of 30 cm,mutant strain YM421-2# was obtained,which exhibited respective increases of melanin yield by 10.03% and biomass by 11.33% when compared with the start strain.This mutant strain can be used for industrial production of melanin as a candidate.