响应面法优化酸水解制备波纹巴非蛤小分子肽工艺

Response Surface Methodology for Optimization of Small Peptide Preparation from Paphia undulate Meat by Acid Hydrolysis

应用响应面分析法优化酸水解制备波纹巴非蛤小分子肽的工艺条件。采用二次正交旋转组合设计试验,以波纹巴非蛤肽得率为响应值,进行3因素5水平的响应面分析,建立二次回归模型,其拟合优度为92.09%。获得的最优酸水解条件为:固液质量比1:3、盐酸浓度6.4mol/L、酸水解温度92℃、酸水解时间5.3h。在此条件下肽得率为82.21%,与模型预测的肽得率84.04%接近。SDS-PAGE电泳测得酸水解液组分的最小分子质量小于2kD。

In this study,an acid hydrolysis procedure for preparing small peptides from Paphia undulate meat was proposed and optimized using response surface methodology（RSM）.A quadratic regression model describing small peptide yield as a function of 3 variables was created based on a 5-level quadratic orthogonal rotation combination design,whose goodness of fit was 92.09%.The optimal hydrolysis conditions were found as follows： raw material-to-water ratio of 1：3,hydrochloric acid concentration of 6.4 mol/L,hydrolysis temperature of 92 ℃ and hydrolysis time of 5.3 h.Under the optimal conditions,the predicted and experimental recovery rates of small peptides were 84.04% and 82.21%,respectively.SDS-PAGE revealed that the smallest peptides in the resulting hydrolysate were less than 2 kD.