摘要
应用基因组改组技术提高耶罗维亚酵母γ-癸内酯的产量。耶罗维亚酵母原生质体在成串脉冲电场频率1000kHz,融合电压强度6kV/cm,脉冲个数4个,成串脉冲电压40V,脉冲宽度20μs的条件下进行电融合,原生质体的存活率能达到61.6%,其中一株改组菌株GDL产量为1.237g/L,是出发菌株产量的8倍。
In the present study, genome shuffling was used to improve γ-decalactone（GDL）pmduction of Yarrowia lipoly6ca. The protoplast of Yarrowia lipolytica was electrofused in transverse alternating field at frequency of 1000kHz and field strength of 6kV/cm. Fusion was irritated by voltage pulse （6kV/cm, 20μs duration, 4 pulse）. The fraction surviving of protoplast was 61.6%. The γ-decalactone production in one of the daughter strains was 1.237g/L, which was 8 times higher than that of the starting stain.
出处
《中国酿造》
CAS
北大核心
2011年第11期97-100,共4页
China Brewing
基金
淮安市科技支撑计划(工业)资助项目(HAG09040)
